Stepped vitrification technique for human ovarian tissue cryopreservation
نویسندگان
چکیده
منابع مشابه
I-10: Vitrification of Baboon Ovarian Tissue:Will Vitrification Replace Conventional Freezing for Ovarian Tissue Cryopreservation?
Background: The aim of this study was to evaluate the efficacy of a vitrification protocol developed by our group to cryopreserve ovarian tissue. For this, we used ovaries from non-human primates in order to have an animal model close to the clinical setting. Materials and Methods: Ovarian biopsies from five adult baboons were vitrified, warmed and autografted. After five months, follicle survi...
متن کاملVitrification versus conventional cryopreservation technique
During in vitro fertilization treatment (IVF), a surplus of oocytes is produced which results in a surplus of embryos which must be preserved. Due to the recent tendency in ART treatment to transfer fewer embryos in order to avoid multiple pregnancies, partly a result of new laws and guidelines aimed at controlling the number of embryos to be transferred and the number of oocytes to be fertiliz...
متن کاملVitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue.
BACKGROUND Controlled-rate freezing of ovarian cortical tissue for preservation of fertility among young women facing chemo- or radio-therapy is a widely accepted procedure. To improve the method for cryopreservation of ovarian tissue, particularly the stroma, we carried out a systematic comparison of vitrification versus slow programmed freezing. METHODS Ovarian tissue from 20 women, donated...
متن کاملChapter 13 Human Ovarian Tissue Vitrification.
Ovarian freezing and transplantation has garnered increasing interest as a potential way of preserving fertility in cancer patients as well as for women who just wish to delay childbearing. This chapter spells out our techniques of ovarian cortex vitrification and results for frozen compared to fresh ovarian cortex transplantation (in one single series from one center for the sake of consistenc...
متن کاملHuman ovarian tissue: vitrification versus conventional freezing.
Sir, In the challenging paper by Wang et al. (2008), the authors have reported about an effective method of vitrification of human ovarian tissue with direct contact of cells in liquid nitrogen, named needle immersed vitrification. Childbirth after cryopreservation of ovarian tissue is now a reality (Donnez et al., 2004; Demeestere et al., 2007; Meirow et al., 2007; Andersen et al., 2008) and t...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2019
ISSN: 2045-2322
DOI: 10.1038/s41598-019-56585-7